3D CoSeedis™ from abc biopply ag is a novel scaffold-free 3D cell co-culture system. It consists of a unique conical agarose matrix array (CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth. Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardisation of protocols.
Contact or distance co-cultures (fully separable) ● Serum, serum-reduced or serum-free conditions possible
Allows long term 3D culture of cell aggregates ● Sufficient supply with nutrients, oxygen, and catabolites – efficient disposal of toxic waste products ● Efficient use of physically separated feeder cells (distance co-culture) ● Cultivation in standard or defined media
Applicable to various cell types (see Applicable Cell Systems below) ● Supports the formation of aggregates of spheroidal and non-spheroidal cell types ● Highly reproducible cell aggregates ● Suitable for high-throughput applications
These cells have been tested sucessfuly for aggregate sphere formation by our accademic partners.
Details are described in the publication: A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement Andreas R. Thomsen et al., Lab Chip, DOI 10.1039/C7LC00832E.
The modular composition of the 3D system allows distance co-cultures and, consequently, the standardisation of protocols. abc biopply’s product portfolio contains already standardised protocols for a wide variety of co-cultures, especially for the cultivation of different tumours.