3D CoSeedis™ is a novel scaffold-free 3D cell co-culture system that offers unique benefits in terms of standardisation, reproducibility and reliability.

The modular setup ensures, the system can be adapted to a broad range of different applications. The following systems are available:

Starter Kits

Offers an easy access point to the 3D CoSeedis™ System. It contains all required material and protocols to successfully setup organoid cultures.

The following components are included:

  • 6 matrices, available in 1 of 2 formats:
    • 880 microwells per matrix (1 x 1)
    • 200 microwells per matrix (2 x 2)
  • Spatula (matrix handling)
  • Two 6-well plates for organoid cultures
  • Cell Strainer
  • Hemocytometer

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Co-Culture Systems - 6 matrices

The 3D CoSeedis Co-Culture System is an innovative technology that allows the generation of organoids in presence of feeder cells. The system offers a unique way to keep feeder and test cells physical separated.

The following components are included:

  • 6 matrices, available in 1 of 2 formats:
    • 880 microwells per matrix (1 x 1)
    • 200 microwells per matrix (2 x 2)
  • Two 6-well plates for organoid cultures
  • Cell Strainer
  • Hemocytometer


Alternatively, organoids can be grown in the absence of feeder cells. Please ask for the according protocol (Organoid Standard System).


Note: The system DOES NOT contain a spatula.


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Co-Culture Systems - 72 matrices

The 3D CoSeedis Co-Culture System is an innovative technology that allows the generation of organoids in presence of feeder cells. The system offers a unique way to keep feeder and test cells physical separated.

The following components are included:

  • 72 matrices, available in 1 of 2 formats:
    • 880 microwells per matrix (1 x 1)
    • 200 microwells per matrix (2 x 2)
  • 24 6-well plates for organoid cultures
  • 12 Cell Strainer
  • Spatula


Alternatively, organoids can be grown in the absence of feeder cells. Please ask for the according protocol (Organoid Standard System).



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Micro-Organoid Histology System

Offers the complete workflow from cells to organoides and contains instructions and all components for their histological analysis.

It consists of:

  • 6 matrices, available in 1 of 2 formats:
    • 880 microwells per matrix (1 x 1)
    • 200 microwells per matrix (2 x 2)
  • Spatula (matrix handling)
  • Two 6-well plates for organoid cultures
  • PBS w/o Mg2+/Ca2+
  • Histology cassettes
  • Low melting point agarose
  • Cell Strainer
  • Hemocytometer


Note: The system DOES NOT contain formalin (nor any other fixatives).


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Micro-Tumour Systems

A standardised system to reliably grow micro tumours in a highly reproducible fashion. Workflows include pre-validated protocols for their analysis (e.g. volumetric size measurements to determine tumour growth). All Micro-Tumour Systems contain a pre-tested tumour cell line as well as the according optimised media, buffers etc.:

  • 6 matrices, available in 1 of 2 formats:
    • 880 microwells per matrix (1 x 1)
    • 200 microwells per matrix (2 x 2)
  • One of the following cell lines:
    • Colon: WiDr
    • Liver: Huh-7 -> in preparation
  • Spatula (matrix handling)
  • 3D CoSeedis™ cell culture medium
  • 3D CoSeedis™ cryo-medium
  • PBS w/o Mg2+/Ca2+
  • Cell Strainer
  • Hemocytometer


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Single Cell Analysis Systems

Offers a pre-validated and standardised system to extract single cells from previously grown micro organoids. Besides all organoid culture relevant components, this system also provides a proprietary organoid dissociation solution.

 

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Customised Systems

Profit from abc biopply’s extended experiences in the standardisation of 3D CoSeedis™ applications. Our customised systems will implement your cell line of choice into the 3D CoSeedis™ platform. Please contact us to discuss your requirements with us.

 

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