New standards in 3D Co-Culture
3D CoSeedis™ from abc biopply is a novel scaffold-free 3D cell co-culture system. It consists of a unique agarose-based matrix containing an array of conical microwells. The particular and proprietary topography of this arrangement allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume, hence cell growth. Furthermore, the modular composition of the 3D CoSeedis™ system allows co-culturing of feeder cells that are physically separated from the actual test cells (distance co-culture). Consequently, this set-up counts responsible for the standardisation of protocols under defined conditions.
Contact or distance co-cultures with feeder cells ● Combining up to 3 cell types to analyse interactions ● Serum, serum-reduced or serum-free conditions possible ● Application of tissue specific ECM components
Agarose-based Permeable Matrix
Allows long term 3D culture of cell aggregates ● Sufficient supply with nutrients, oxygen, and catabolites – efficient disposal of toxic waste products ● Efficient use of physically separated feeder cells (distance co-culture) ● Cultivation in standard or defined media
Applicable to various cell types ● Supports the formation of spheroidal and non-spheroidal aggregates ● Suitable for high-throughput and high-content applications
Process Directed Workflow Protocols
Validated protocols ● Standardised and optimised components ● Highly reproducible 3D cell constructs ● Suitable for high-throughput and high-content applications
|Aggregation of non-spheroidal cells||-||+|
|Long term culture||-||+|
|Easy harvesting of spheroids||+||+|
|HTS & HCS compatibility||- / +||+|
|Fully validated workflows/systems||-||+|
These cells have been tested successfully for aggregate spheroid formation by our academic partners.
Details are described in the publication: A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement Andreas R. Thomsen et al., Lab Chip, DOI 10.1039/C7LC00832E.
The modular composition of the 3D CoSeedis ™ system allows distance co-cultures and, consequently, the standardisation of protocols. abc biopply’s product portfolio contains already standardised protocols for a wide variety of co-cultures, especially for the cultivation of different tumours.
Various application notes have been developed that constantly extend the range of assays in which 3D CoSeedis™ has been successfully implemented. Check out the latest notes: