3D CoSeedis™ easy microscopy and in-chip imaging of 3D spheroids/organoids - from life-imaging to live/dead™ assays
The analysis of 3D spheroids/organoids can be challenging. Particularly image capturing of 3D spheroids/organoids in an automated and high-throughput context can be cumbersome and time consuming. With 3D CoSeedis™, spheroids/organoids have become assessable for automated image acquisition, both in terms of 3D life-imaging and endpoint analysis. 3D CoSeedis™ makes automated high-throughput imaging possible through:
Transparent, non-fluorescent chip matrix allows in-chip imaging of spheroids/organoids
Unique 3D CoSeedis™ chip design fixes all spheroids/organoids in a defined positions relative to each other
Defined positions of spheroids/organoids in the same focal plane (z-axis) allows easy automated image capture
In-chip staining protocols allow whole-mount immunofluorescence or immunohistochemistry
In-chip live/dead™ staining protocols allow easy assessment of spheroid/organoid viability
The splitting of 3D-CoSeedis™ chips enables the simultaneous analysis of spheroids/organoids in different downstream analytical procedures, e.g. imaging, IHC, RNA profiling.
But the possibilities in high-content analysis of spheroids/organoids with 3D CoSeedis™ go much further. For example, the splitting of 3D-CoSeedis™ chips enables the simultaneous analysis of spheroids/organoids in different downstream analytical procedures, e.g. imaging, IHC, RNA profiling. The advantage in this approach is evident: for all types of analyses, the spheroids/organoids are grown under identical conditions and have been subjected to the same treatments, thus the represent biological replicates giving the analyses statistical relevance.
Are you interested to get to know more about the numerous validated analysis applications with 3D CoSeedis™? Visit the application section on our website to download relevant white papers and application notes.