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3D CoSeedis™ in extracellular vesicle production

A new publication by Liliia Paniushkina, Elena Gueso-Navarro, and Xu Cheng from the research group of Irina Nazarenko from the University of Freiburg, Germany, has just shown the remarkable benefits 3D CoSeedis™ offers in the production of extracellular vesicles (EV) from 3D cultures. In this article, published in October 2020 in Methods of Enzymology, they give in depth insight in the use of various 3D systems in the preparation of EVs and underline the outstanding performance and advantages 3D CoSeedis™ offers in comparison to other methods. Advantages include:
  • Long term culture
  • Homogenous and controlled size of cell aggregates
  • Controlled cell number and viability
  • High reproducibility
  • Applicability to various primary cells and cell lines
Paniushkina et al., Three-dimensional cell models for extracellular vesicles production, isolation, and characterization; p215, Oct 2020
From: Three-dimensional cell models for extracellular vesicles production, isolation, and characterization
Liliia Paniushkina, Elena Grueso-Navarro, Xu Cheng, Irina Nazarenko
Methods in Enzymology, October 2020; https://doi.org/10.1016/bs.mie.2020.09.005
The article gives detailed instructions and lists protocols on how to use 3D CoSeedis™ in EV production, isolation and analysis. Presenting well-structured and useful decision trees and schematic representations of the workflow, the authors describe how 3D Coseedis™ can be used to collect highly comparative samples of EVs from various cell types (primary cells, stem cells, and cell lines). Furthermore, they also define what the critical parameters are in such an experimental set-up.
 
The group of Irina Nazarenko further shows that cells grown under 3D conditions produce substantially higher amounts of vesicles of different sizes which strongly supports the application of 3D models in EV studies.
This is in accordance with previously published observations by Sara Rocha et al., Adv. Sci., 2018 (read publication here), in which EVs from 3D models not only showed a different size distribution to those collected from cells in 2D but also contained a different load of microRNAs and proteins. The authors of this study therefore conclude that the production of EVs in 3D systems has a significant impact on the biogenesis and cargo of EVs and lead ultimately to physiologically more relevant results.
-> DOWNLOAD PUBLICATION
Could I spark you interest? Please let me know if you want to hear more about the use of 3D CoSeedis™ in EV production.

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