Mass density analysis reveals: 3D CoSeedis™ outperforms conventional 3D culture systems in terms of homogeneity and uniformity
In a collaboration between the Italian company CELLdynamics and abc biopply, spheroids from the colorectal andenocarcinoma cell line HT-29 were generated in the 3D CoSeedis™ 3D cell culture system. The results clearly showed superior performance of 3D CoSeedis™ to conventional alternative 3D culture systems. The resulting spheroids showed
lower variance in diameter
lower variance in weight
significantly higher mass density
than comparable spheroids generated in ultra-low attachment plates. These findings emphasise the unique features of 3D CoSeedis™ in the generation of highly homogenous and uniform spheroids in large quantities. They define yet another piece of evidence for the unsurpassed suitability of 3D CoSeedis™ for HTS compound testing.
These results indicate:
Higher compactness of cells in HT-29 spheroids
Close resemblance to observed tumour in vivo situation
Higher potential to mimic physiologically important gradients, such as nutrients, osygen, waste products, etc.
Furthermore, a recent study (download) by A. Sargenti et al. shows a clear correlation between spheroid structure and mass density and the response to NK cell-mediated cytotoxicity. The authors accentuate the importance of standardised culture conditions in order to receive highly homogenous and uniform spheroids for anti-cancer drug testing.
