PART 1: Communication via direct cell-to-cell contacts vs. communication via soluble factors (distance co-culturing)
Revolutionize predictive disease modeling. A brief introduction to highly efficient and predictive disease modelling with 3D CoSeedis™. PART 1
Many diseases elude current in vitro testing methods and consequently make the identification of effective new treatments cumbersome if not impossible. Likewise, in vitro diagnostics are often difficult or lead to ambiguous results. With 3D CoSeedis™, abc biopply is now introducing arevolutionary 3D cell culture platform that enables to address complex disease models at an unprecedented depth. Yet, the 3D CoSeedis™ system is simple, robust and user friendly in its set-up, highly reproducible and reliable.
For the first time, 3D organoids/spheroids are now:
Accessible in complex cell-to-cell and
Organ-to-organ communication systems
Available in high quantities
Highly homogeneous and uniform for reliable read-outs
3D CoSeedis™ therefore:
Optimises the statistical biological relevance of test results
Makes mimicking of a variety of different cell communication models possible
Opens a new era of disease modelling
PART 1: Communication via direct cell-to-cell contacts vs. communication via soluble factors (distance co-culturing)
Feeder cells are often used
To support test cells of interest during their aggregation phase
To receive assessable 3D structures, particularly with cells that do normally not form 3-dimensional constructs in vitro
Contact: feeder cells are intermingled with test cells to form 3D aggregates Distance: feeder cells are separated from test cells by 3D CoSeedis™ chip. Chip matrix permeable to all relevant factors.
Using regular technologies, such as hanging drop or liquid overlay, the only way to make use of feeders is to mix them with test cells.
Mixed cultures of test- and feeder cells result in:
Intermingled and inseparable 3D constructs consisting of test cells and feeders
Biased results due to mixed response signals to test compound
Why does 3D CoSeedis™ pose such a revolutionary new way to do 3D cell culture?
3D CoSeedis™ circumvents the drawbacks of regular 3D cultures by introducing the distance co-culture approach to use feeder cells:
An elegant and easy way to use feeders physically separated from test cells
It allows supporting factors from the feeder cells to still influence the test cells
Feeders can be left behind after aggregation by simply lifting off the 3D CoSeedis™ chip including the test cell 3D cultures
Subsequently, 3D cultures can be grown further in a fresh culture plate in the absence of feeder cells
One last thing...
... the distance co-culture setup of 3D CoSeedis™ can do a lot more:
It also allows to mimic cell-to-cell communication in general
It distinguishes between membrane-bound signal transduction (contact co-culture) and communication pathways mediated via soluble factors (distance co-culture)
And it can be used to mimic complex, multi-cellular disease models….. more about that in Part II of this exciting story
Are you curious and want to know more about the many possibilities 3D CoSeedis™ offers to address complex disease models? Follow our next mailing on PART 2 in disease modelling addressing the communication between multiple 3D structures. In the meantime, do not hesitate to contact me to discuss the various features of 3D CoSeedis™ in complex disease modelling with me. I am looking forward to hearing your feedback.
Best regards
Marco Leu, PhD Sales & Operation abc biopply ag Direct Email / Phone +41 (0)79 834 95 56
