References

  • Three-dimensional cell models for extracellular vesicles production, isolation, and characterization
    Liliia Paniushkina, Elena Grueso-Navarro, Xu Cheng, Irina Nazarenko, https://doi.org/10.1016/bs.mie.2020.09.005

  • Tspan8 is expressed in breast cancer and regulates E-cadherin / catenin signalling and metastasis accompanied by increased circulating extracellular vesicles
    Maren Vogelstaetter et al., J. Pathol., 2019, DOI:10.1002/path.5281

  • 3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles
    Sara Rocha et al., Adv. Sci. 2018, 1800948; DOI: 10.1002/advs.201800948
  • A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
    Andreas R. Thomsen et al., Lab Chip, 2018, 18, 179; DOI: 10.1039/c7lc00832e
  • Proteome Profiling of Primary Pancreatic Ductal Adenocarcinomas Undergoing Additive Chemoradiation Link ALDH1A1 to Early Local Recurrence and Chemoradiation Resistance
    V. O. Oria et al., Translational Oncology, Vol. 11, Issue 6, Dec. 2018; DOI.org/10.1016/j.tranon.2018.08.001
  • A binary approach to the colony forming assay: reliable and reproducible read-outs using 3D CoSeedis™
    Marco P. Leu et al., Download Whitepaper
  • Easy mass production of homogenous and uniform 3D spheroids for high-throughput screening applications
    Marco P. Leu et al. Download Whitepaper

3D CoSeedis™ convinces through its innovative modularity that allows us to grow cells in 3D aggregates that normally wouldn’t do so. It either supports aggregation through its unique topography or by the ability to grow feeder cells in a physically separated, distant manner. When investigating the response rate of various tumour cells to radiation, the 3D CoSeedis™ System allows us the flexibility and statistics that we need in experiments.

Andreas Thomsen, MD. dept of Radiation Oncology
University Medical Center Freiburg, Germany

3D CoSeedis™ is ideally suited to isolate extracellular vesicles (EV) for analytic and functional characterisation from the 3D cell culture of cell lines as well as primary cells. Compared to 2D, the 3D environment is likely to trigger changes in EV cargo, such as increased amounts of certain miRNAs while at the same time showing decreased amounts of their target proteins, which may affect EV biological function. 3D CoSeedis™ allows high yields of EVs/cell  in a cost- and effort-efficient manner. 

PD Dr. Irina Nazarenko
Unversitätsklinikum Freiburg, Germany

3D CoSeedis™ offers the possibility to produce and collect exosomes in conditioned media from up to 900 spheroids per treatment in an easy and reliable manner. The unique topography of the 3D CoSeedis™ chip further allows the aggregation of cells that would not do so in liquid overlay nor in hanging drop systems and therefore substantially extends the range of conditions media we can work with.

Dr. Peter Frost
CEO and Owner of FROST LIFESCIENCE, Germany

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