3D CoSeedis™ offers the possibility to produce and collect exosomes in conditioned media from up to 900 spheroids per treatment in an easy and reliable manner. The unique topography of the 3D CoSeedis™ matrix further allows the aggregation of cells that would not do so in liquid overlay nor in hanging drop systems and therefore substantially extends the range of conditions media we can work with.

3D CoSeedis™ is ideally suited to isolate extracellular vesicles (EV) for analytic and functional characterisation from the 3D cell culture of cell lines as well as primary cells. Compared to 2D, the 3D environment is likely to trigger changes in EV cargo, such as increased amounts of certain miRNAs while at the same time showing decreased amounts of their target proteins, which may affect EV biological function. 3D CoSeedis™ allows high yields of EVs/cell  in a cost- and effort-efficient manner.  

So far, I can tell that we are very satisfied. We tried several other tools for spheroid culturing, but this is by far the easiest to handle.

3D CoSeedis™ convinces through its innovative modularity that allows us to grow cells in 3D aggregates that normally wouldn’t do so. It either supports aggregation through its unique topography or by the ability to grow feeder cells in a physically separated, distant manner. When investigating the response rate of various tumour cells to radiation, the 3D CoSeedis™ System allows us the flexibility and statistics that we need in experiments.

3D CoSeedis™ convinces through its innovative modularity that allows us to grow cells in 3D aggregates that normally wouldn’t do so. It either supports aggregation through its unique topography or by the ability to grow feeder cells in a physically separated, distant manner. When investigating the response rate of various tumour cells to radiation, the 3D CoSeedis™ System allows us the flexibility and statistics that we need in experiments.