09.01.2019 - Extracellular Vesicles Composition Affected by 3D Cell Architecture
3D CoSeedis™ has recently been used to demonstrate the effect of 3D cellular architecture on microRNA and protein cargo of extracellular vesicles (see https://doi.org/10.1002/advs.201800948).
In this study, 3D CoSeedis™ has been used to address the impact of cellular architecture on extracellular vesicle content and function. To do so, the authors used comparative analysis of biochemical features, RNA and proteomic profiles of vesicles secreted by both, 2D and 3D cultures of gastric cancer cells.
The 3D CoSeedis™ system was used to grow those cells in a high-density 3D array, which allowed the collection of conditioned media containing high numbers of secreted extracellular vesicles for analysis. The paper shows that 3D CoSeedis™ presents a robust experimental system to efficiently produce extracellular vesicles under controlled conditions and closer to the original physiological environment of the cells. Specifically, the authors showed significant differences in extracellular vesicle composition with respect to microRNAs and consequently a downregulation of their corresponding target proteins.