The following short movie shows how efficiently and easy 3D constructs can be harvested from the 3D CoSeedis™ matrix. A few simple steps allow the collection of up to 880 fully viable constructs per matrix for downstream applications (e. g. DNA, RNA, protein extractions, whole mount staining, replating, etc.).
The following tutorial shows the transfer of a 3D CoSeedis™ matrix from one well to another. It is shown in both, the 6-well and the 24-well plate.
This easy procedure is used:
1.) After initial seeding of matrices
2.) In feeder co-culture experiments
3.) Media change or washing steps (optional)
For more details, also consult our details protocols.
abc biopply’s latest tutorial shows how to seed cells into both, the 6-well and the 24-well format of 3D CoSeedis™. Detailed protocols are available here.
abc biopply also provides a calculation tool to make cell dilutions easy and fast. It can be downloaded for free here.